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1st workshop on QuantitaTive microscopy for studying membrane dynamics
June 11-12, Braga, Portugal
AGENDA
Program
Day 1: June 11
09:30
10:45
12:00
14:00
15:45
ER-PM Contacts: Principals of Membrane Dynamics
Chris Stephan (University College London)
Differential lipid composition and regulation along the hippocampal longitudinal axis
Tiago Gil Rodrigues (Medical School, University of Minho)
Lunch
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Fluorescence Dynamics Techniques
Camilo Guzmán (INL)
Insights into Nutrient Transporters endocytosis
Sandra Paiva (CBMA, University of Minho)
Day 2: June 12
09:30
10:45
12:30
14:30
Phosphoinositide Lipid Switches in Endocytosis
Chris Stephan (University College London)
Practical 1
Chris Stephan (University College London)
Lunch
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Practical 2
Chris Stephan | Sandra Paiva | Camilo Guzmán
PALESTRANTES
Organizing Commite
Sandra Paiva (Chair)
University of Minho (Portugal)
Camilo Guzmán
INL
Chris Stephan
(Co-chair)
University College London
Pieter de Beule
INL
Alexandra Gonçalves
University of Minho (Portugal)
Cláudia Barata
University of Minho (Portugal)
Rosana Alves
University of Minho (Portugal)
MAPA
Location
INL (International Iberian Nanotechnology Laboratory – INL)
Avda. Mestre José Veiga s/n, Braga
Organizing institutions
Sponsors
RSVP
Scope of the workshop
This workshop will address fluorescence dynamic techniques that are used to quantify movement and dynamic events (i.e. diffusion, interactions, etc.) within the cell. It will also cover fluorescence correlation spectroscopy (FCS), fluorescence lifetime imaging microscopy (FLIM), Forster Resonant Energy Transfer (FRET) and Fluorescence Recovery After Photobleaching (FRAP). We will also focus on protein trafficking, including ER-PM contacts in endocytosis and PIPs and PIP conversion events in driving distinct stages of endocytosis. The relevance of these topics in Biomedicine will be highlighted.
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